Extracellular cadmium in the bronchoalveolar space of long-term tobacco smokers with and without COPD and its association with inflammation
نویسندگان
چکیده
Tobacco contains cadmium, and this metal has been attributed a causative role in pulmonary emphysema among smokers, although extracellular cadmium has not to date been quantified in the bronchoalveolar space of tobacco smokers with or without COPD. We determined whether cadmium is enhanced in the bronchoalveolar space of long-term tobacco smokers with or without COPD in vivo, its association with inflammation, and its effect on chemokine release in macrophage-like cells in vitro. Bronchoalveolar lavage (BAL), sputum, and blood samples were collected from current, long-term smokers with and without COPD and from healthy nonsmokers. Cadmium concentrations were determined in cell-free BAL fluid using inductively coupled plasma mass spectrometry. Blood monocyte-derived macrophages were exposed to cadmium chloride in vitro. Depending upon the type of sample, molecular markers of inflammation were quantified either as protein (enzyme-linked immunosorbent assay) or as mRNA (real-time polymerase chain reaction). Cadmium concentrations were markedly increased in cell-free BAL fluid of smokers compared to that of nonsmokers (n=19-29; P<0.001), irrespective of COPD. In these smokers, the measured cadmium displayed positive correlations with macrophage TNF-α mRNA in BAL, neutrophil and CD8(+) cell concentrations in blood, and finally with IL-6, IL-8, and MMP-9 protein in sputum (n=10-20; P<0.05). The cadmium chloride exposure caused a concentration-dependent increase in extracellular IL-8 protein in monocyte-derived macrophages in vitro. In conclusion, extracellular cadmium is enhanced in the bronchoalveolar space of long-term smokers and displays pro-inflammatory features. Its pathogenic role in tobacco-induced disease deserves further evaluation.
منابع مشابه
Impact of tobacco smoking on cytokine signaling via interleukin-17A in the peripheral airways
There is excessive accumulation of neutrophils in the airways in chronic obstructive pulmonary disease (COPD) but the underlying mechanisms remain poorly understood. It is known that extracellular cytokine signaling via interleukin (IL)-17A contributes to neutrophil accumulation in the airways but nothing is known about the impact of tobacco smoking on extracellular signaling via IL-17A. Here, ...
متن کاملInterleukin-16-producing NK cells and T-cells in the blood of tobacco smokers with and without COPD
BACKGROUND Long-term exposure to tobacco smoke causes local inflammation in the airways that involves not only innate immune cells, including NK cells, but also adaptive immune cells such as cytotoxic (CD8+) and helper (CD4+) T-cells. We have previously demonstrated that long-term tobacco smoking increases extracellular concentration of the CD4+-recruiting cytokine interleukin (IL)-16 locally i...
متن کاملHomocystein Level and Total Antioxidant Capacity in Chronic Obstructive Pulmonary Disease
Abstract Background and Objective: Oxidant-antioxidant imbalance plays a key role in pathogenesis of chronic obstructive pulmonary disease (COPD). This study aimed to evaluate homocystiene and total antioxidant capacity in COPD patients, compared to smoker and non-smoker healthy people. Material and Methods: We measured total antioxidant capacity with Cayman Kit, uric acid with Pars Azm...
متن کاملThe effect of metal deposition on antioxidant enzymes of lens in smokers of Karachi, Pakistan
Background: Blindness due to cataract is a major and important problem in Pakistan. The problem is expected to increase in future due to increase in tobacco smoking. The aim of study was to look for the mechanism associated with metal deposition and its effect on antioxidant enzymes in lens of smokers in Karachi, Pakistan. Methods: 20 cataract patients were randomly selected from Out Patient De...
متن کاملThe Concentration of Nicotine in Indoor Air of the Cafes of Tehran and the Biological Levels of Nicotine and Cotinine in Exposed Smokers in These Cafes
Background & objectives: The number of waterpipe cafes has considerably grown in Tehran and other cities of Iran. This increase has occurred due to a wrong belief that the use of hookahs is harmless and relatively safe. So, the aim of current study is to investigate the nicotine concentration in the indoor air of the waterpipe and cigarette cafés as well as the biological concentration of nicot...
متن کامل